CHO, or the Chinese Hamster Ovary cell line, is currently the most popular choice for mass production of recombinant protein therapeutics. They are robust in culture and can produce a variety of recombinant glycoproteins in large scale at high titers. Different CHO clones often possess diverse nutritional requirements that are unique to each clone, and this is an important consideration when we select for the most suitable media. In our Knowledge Culture Basic Education Series on CHO media, we will explain about CHO cells: their lineage, historical culture media, and process improvements. Next, we will explain basic media components and functions, media for different culture modes, media selection criteria and pathways, and some examples of current media offerings. We will also show the manufacturing workflows for different media formats and briefly discuss about establishing supply redundancies. Finally, we will close off with a case study on CHO fed-batch platform process.
Learning Objectives
- History of CHO culture media and process improvements
- Basic media components and functions
- Media for different culture modes and media selection criteria and pathways
- Example of CHO media offerings and media manufacturing and supply redundancies
- Case study (Fed-batch platform process)
Speaker
Siow Leng (SL) Sim, PhD
PhD degree in Chemical and Biomolecular Engineering
Senior Manager, Field Applications, Thermo Fisher Scientific
Siow-Leng (SL) Sim is currently Field Applications Sr Manager with Thermo Fisher Scientific BioProduction Group. Based out of Singapore, he manages Field Application Scientist (FAS) teams in Asia Pacific and Japan region. Previously he served R&D, manufacturing and commercial support roles in both global biotech companies and start-ups. Mr. Sim graduated with B.Eng, M.Eng and Ph.D. in Chemical and Biomolecular Engineering from the National University of Singapore.