Cell line creation is an important part of biologics development. Transient expression cell lines are not suitable for typical bioprocessing, such as monoclonal antibodies (mABs), because of the variability in the production and quality of the target substance, the risk to the safety of the cell line, and compliance with various regulations. Developing high-producing, stable cell lines is fundamental to the production of biotherapeutics such as monoclonal antibodies, bi-specific monoclonal antibodies, fusion proteins, and vaccines.
In today’s Knowledge Culture Basics Education Series on cell line development, we will introduce what is cell line development and why we need it? Then, a typical cell line development process with the outline of vector construction, transfection, single cell cloning, as well as industry trends will be explained. Lastly, we would like to describe a case study of cell line development in collaboration with a customer.
Learning Objectives
- What is cell line development? Why do we need it?
- Overall cell line development process
- Industry trends
- Case study using Gibco Freedom CHO-S kit
Speaker
Yusuke Watamura
Field Application Scientist, Cell Culture and Cell Therapy, BioProduction, Thermo Fisher Scientific Japan
Yusuke Watamura is a Field Application Scientist responsible for the technical and application support of Cell Culture and Cell Therapy for Japan based in Tokyo. Prior to joining the Field Application Scientist (FAS) team, he was a Sr. sales representative responsible for molecular biology products (Invitrogen brand), a bioproduction account manager responsible for the management of key accounts, and technical sales specialist responsible for Gibco cell culture products; he has been working with Thermo Fisher Scientific for 16 years. He graduated from the Tokyo University of Agriculture in 2003 with a bachelor’s degree in Bioscience and in 2005 with a master’s degree in Agriculture (Molecular biology and Microbiology).