Learn how restriction endonucleases were developed for use in the laboratory. Review the factors that influence optimal DNA digestion, common troubleshooting issues, and new applications for restriction enzymes in molecular biology workflows.

Learning objectives:

• Learn why restriction enzymes were created
• Learn how to optimize DNA digestion
• Explore restriction enzyme applications and troubleshooting

Webinar content:

• Discovery and historical perspective
• Highlights of DNA cutters: types, terms, nomenclature
• Features: what matters most
• How to control digestion reactions and cut DNA with confidence
• Classic applications
• Novel applications, type IIs cloning